algae – nitrogen test: colour scale

14.11.23 Experiment right up:

I cut my plastic bags to chair and heats divided the sections, labelling each compartment with the amount of nitrogen + my name, the date and the strain of algae
I measuring out the different sodium nitrate amounts in 6 little bottles (choosing sodium nitrate because it’s the nitrogen source in grow medium)
I took the ratio of nitrogen within the BBM grow medium as my point of reference – 25g per litre – and used this as the degree to increase by. (1.25g in my 50ml mixture)
I added 10ml of water to the bottles to dissolve the sodium nitrate salt before putting them all in the autoclave (with slightly unscrewed lids) along with 400ml of water and my measuring 50ml beaker – all intended to be sterilised for 40 mins
Got the algae from the drive and the BBM grow medium in the flow hood. I used a mechanic pipet where you could control the exact amount of liquid – so that I could add 1ml (1000) of the BBM into the measuring beaker, then poorer the first container (always go from least strong to most to avoid fucking up milder quantities) and then fill the beaker up to the 50ml mark with the sterile water and add mixture to the first compartment using funnel
Repeat with the rest
Then use the larger, pistol pipet to get 6ml of algae and add to each compartment